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2007 Annual Report

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Core Services Core

Core Services Core laboratories at Van Andel Institute (VAI) provide our researchers and external customers with several services essential to the research process. Having these services available on site helps to streamline the research process. Our core services include: Analytical, Cellular, and Molecular Microscopy (Imaging, Histology and Diagnostic Pathology): pathology, tissue resources, paraffin, frozen and plastic tissue processing, Vibratome processing/sectioning, laser capture micro-dissection, laser scanning confocal imaging, in vivo intravital imaging, digital imaging, spectral imaging and deconvolution Antibody Production: monoclonal and polyclonal antibody production, purification (Protein-G affinity column, PBS), characterization (IgG, IP, Western, IFA), immunization, serum screening, fusion, recloning, expanding, freezing and growing hybridoma cell lines, lyophilization, immunolabeling, affinity column preparation, bi-specific mAbs Computational Biology: custom data analysis and consulting, assistance with computationally intensive tasks, writing of niche programs that perform data-specific tasks not commonly found in existing commercial software, assistance in using sequence analysis tools and gene expression microarray analysis programs Flow Cytometry: 12-Color BD FACSAria High- Speed Cell Sorters, 2 4-Color BD FACSCalibur Cell Sorter, 96/384 Well HTS Autosampler, NPE Cell Analyzer, CBC with differential, blood chemistry profile, data analysis, Shandon Cytospin 3 Germline Modification and Cytogenetics: electroporation of ES Cells, Clone Selection, and DNA Isolation, thawing and expansion of ES cell clones, electroporation of Cre recombinase, microinjection of blastocysts, tail biopsy, DNA isolation, preparation of mouse embryonic fibroblasts, fluorescent in-situ hybridization (FISH), spectral karyotyping (SKY), transgene localization, routine karyotyping (G-banding), chromosomal breakage studies, mouse embryonic stem (ES) cell trisomy 8 screening, mouse embryo cryopreservation, marker-assisted selection breeding using SNP array (Illumina BeadStation), genomic background verification (inbred strains only) Mass Spectrometry and Proteomics: label-free LC-MS protein expression profiling, protein identification from gels and solution, protein molecular weight determination, protein characterization by peptide mapping Microarray Technology: agilent oligonucleotide gene expression microarrays (customizable), ArrayCGH, miRNA expression microarrays, microarray data analysis, RNA isolation and amplification, RNA integrity analysis, DNA isolation and size/concentration analysis, spotted cDNA microarray printing, custom protein microarray printing Sequencing: plasmid, single-stranded templates, cosmids/PACs/BACs, lambda phage DNA, ABI 3700-capillary electrophoresis DNA sequencer, applied Biosystems BigDye sequencing kit, Sephadex G-50 spin column plates for excess dye removal Vivarium: mouse transgenics, transgenics microinjection services, tail biopsies, mouse repository services Xenotransplantation: over- or underexpress gene(s) in established cell lines to validate their role in the etiology of tumors and their malignant progression, analysis and testing of therapeutics Basic Science George Vande Woude, Ph.D. VARI Director and Distinguished Scientific Investigator Laboratory of Molecular Oncology Focuses research on the protein Met, which has been found to be overexpressed in most solid tumors. Animal Imaging: Laboratory of Noninvasive Imaging and Radiation Biology Develops models and partnerships to address problems in medical imaging and radiation exposure, advance imaging and radiation technology, and improve systems and care. Art Alberts, Ph.D. Senior Scientific Investigator Laboratory of Cell Structure and Signal Integration Studies structures that allow cells to change shape and move, important to understanding how cancer spreads. Brian Haab, Ph.D. Senior Scientific Investigator Laboratory of Cancer Immunodiagnostics Investigates protein and carbohydrate abnormalities in cancer patients’ blood and their potential clinical usefulness and the role of protein secretions in cancer progression. Nick Duesbery, Ph.D. Deputy Director for Research Operations and Senior Scientific Investigator Laboratory of Cancer and Developmental Cell Biology Uses non-lethal components of anthrax to develop a new way to fight cancers and learn more about tumor growth and blood vessel formation. Van Andel Research Institute

Labs & Investigators Our laboratories and scientific investigators are dedicated to developing a new understanding of cancer that will help to prevent, treat, and cure human disease. Special Programs Jeff MacKeigan, Ph.D. Scientific Investigator Laboratory of Systems Biology Uses RNA interference to study the interaction of molecules within the cell and connect them to Parkinson disease and the onset and spread of kidney and colon cancer. Craig Webb, Ph.D. Scientific Investigator Laboratory of Tumor Metastasis and Angiogenesis Studies how a tumor spreads to distant parts of the body and is involved in several partnerships with the goal of predicting optimal therapies for cancer patients. Jim Resau, Ph.D. Deputy Director of Special Programs; Director, Division of Quantitative Sciences; Distinguished Scientific Investigator Laboratory of Analytical, Cellular, & Molecular Microscopy Provides imaging services and tissue arrays that allow researchers to view several samples of interest more quickly. Kyle Furge, Ph.D. Scientific Investigator Laboratory of Computational Biology Uses mathematical and computer science approaches to help scientists analyze, store, and distribute research data. Cindy Miranti, Ph.D. Scientific Investigator Laboratory of Integrin Signaling and Tumorigenesis Studies how a cell interacts with its surroundings and receives signals from other parts of the body through proteins called integrins in normal vs. cancer cells. Bin Tean Teh, M.D., Ph.D. Director of VARI International; Director of NCCS-VARI Translational Research Laboratory; Distinguished Scientific Investigator Laboratory of Cancer Genetics Works to identify and study the genetic alterations found in hereditary cancers versus those found in sporadic cases, with a special focus on kidney cancer. Steve Triezenberg, Ph.D. VAI Graduate School Dean and Scientific Investigator Laboratory of Transcriptional Regulation Uses herpes simplex virus to explore the mechanisms that turn on genetic “instructions” at the right time, in the right cells, in response to the right signals – and what goes wrong in disease. Michael Weinreich, Ph.D. Scientific Investigator Laboratory of Chromosome Replication Works to understand the process of chromosome replication within cells to find a way to specifically target and stop the division of cancerous cells. Bart Williams, Ph.D. Senior Scientific Investigator Laboratory of Cell Signaling and Carcinogenesis Studies the Wnt family of proteins, their associated molecules and their implications in prostate cancer, bone formation, and osteoporosis. Eric Xu, Ph.D. Distinguished Scientific Investigator Laboratory of Structural Sciences Studies the structures and functions of protein complexes; the structural information can then be used to develop therapeutic agents for human diseases such as cancer and diabetes. Brian Cao, M.D. Senior Scientific Investigator Laboratory of Antibody Technology Produces, purifies, and characterizes antibodies, which can be used to mark or identify specific molecules such as potential drug targets. Greg Cavey, B.S. Scientific Investigator Laboratory of Mass Spectrometry and Proteomics Separates, analyzes, and identifies individual proteins in cells, allowing researchers to find protein differences between normal and cancer cells. Bryn Eagleson, B.S., RLATG Vivarium Director Vivarium and Laboratory of Transgenics Develops, provides, and supports high-quality mouse modeling services for researchers. Pam Swiatek, Ph.D., MBA Senior Scientific Investigator Laboratory of Germline Modification Provides gene-targeting services in order to develop mouse models that help researchers learn more about genetic mutations involved in disease. VARI Labs & Investigators www.vai.org 27

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