Immunostaining of human hepatocyte growth factor/scatter factor (HGF/SF) Formalin-fixed S-114 cells (NIH 3T3 cells transformed with human HGF/SF and its receptor Met) are stained with a rabbit anti-HGF/SF polyclonal antibody with rhodamine conjugate (red) and a mouse mAb (7-2) with FITC conjugate (green). Both antibodies were produced by the Antibody Technology Lab at VARI. The black and white is a Nomarski-DIC (differential interference contrast) image of the cellular detail. The yellow image indicates co-localized staining of the green monoclonal and red polyclonal antibodies. (Hudson, Zhao, Resau, and Cao) 15
A) Clustering of 15 Wilms tumors and clinical information; B) Three-dimensional clustering The clustering of patients (using Pearson’s correlation) is based on global gene expression profiles consisting of median polished data of 5,594 well-measured spots. The tumors approximately clustered into two main groups, with one group consisting of mostly tumors of high stage (stages III and IV) and the other consisting of mostly tumors of low stage (stages I and II). Two patients with high-stage tumors who died of cancer (Wilms 6, 15) and one patient who had recurrence (Wilms 11) were closely clustered together. One patient (Wilms 14), represented by the orange circle in panel B, who had stage II disease but developed recurrence and died of cancer, was clustered with high-stage tumors. (Takahashi and Teh) 16
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