Normal Epithelia Before After Captured Colon Adenocarcinoma Laser capture of normal colon crypts and adjacent adenocarcinoma Laser capture microdissection of normal colon crypts and adjacent adenocarcinoma of the colon, within a single histopathological section from a patient with metastatic colon cancer. Following capture, the small amounts of RNA and protein obtained can be applied to a global genomic and proteomic analysis using Affymetrix gene chips, cDNA microarrays, and mass spectrometry. In this fashion, genes and proteins that are expressed within the different subcompartments of a heterogeneous tumor (epithelia, endothelia, stroma, inflammatory cells, etc.) can be analyzed. This technology provides an excellent means for identifying the key genes and proteins that are associated with tumor progression and will likely yield targets for the future diagnosis and treatment of cancer. (Webb and Monsma) 51
Immunofluorescent analysis of blood vasculature in the eye of an Lrp5-deficient mouse Mutations that inactivate the gene encoding the low-density lipoprotein receptor–related protein 5 (Lrp5) cause the human syndrome osteoporosis pseudoglioma (OPG). Patients suffering from this syndrome develop earlyonset osteoporosis and have vision problems due to inappropriate vascularization of the eye. We have found that Lrp5-deficient mice model both the osteoporotic and eye problems seen in humans. Shown is an immunofluorescence analysis of a paraffin-embedded eye section from an Lrp5-deficient mouse stained with antibodies to Factor VIII (red) and CD31 (green), two molecules associated with endothelial cells. The prominent vascularization is absent in normal mice of this age. (Williams) 52
Established by Jay and Betty Van Andel in 1996, Van Andel Institute is committed to improving the health and changing the lives of current and future generations, through biomedical research and science education.
Van Andel Institute is a tax-exempt 501(c)(3) charitable organization. EIN 52-2000820
Van Andel Institute is committed to making our website’s content accessible and user friendly to everyone. If you are interested in applying for employment with Van Andel Institute and need special assistance or an accommodation to complete our application process, are having difficulty viewing or navigating the content on this website, or notice any content, feature, or functionality that you believe is not fully accessible to people with disabilities, please call the Human Resources Department at 616-234-5100 or email at email@example.com.
Van Andel Institute is an Equal Employment Opportunity (EEO) and Vietnam Era Veterans Readjustment Assistance Act (VEVRAA) Employer. We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability (physical or mental), marital or veteran status, genetic information, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans.