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2002 Scientific Report

  • Text
  • Institute
  • Report
  • Tumors
  • Protein
  • Signaling
  • Michigan
  • Molecular
  • Proteins
  • Laboratory

Normal Epithelia Before

Normal Epithelia Before After Captured Colon Adenocarcinoma Laser capture of normal colon crypts and adjacent adenocarcinoma Laser capture microdissection of normal colon crypts and adjacent adenocarcinoma of the colon, within a single histopathological section from a patient with metastatic colon cancer. Following capture, the small amounts of RNA and protein obtained can be applied to a global genomic and proteomic analysis using Affymetrix gene chips, cDNA microarrays, and mass spectrometry. In this fashion, genes and proteins that are expressed within the different subcompartments of a heterogeneous tumor (epithelia, endothelia, stroma, inflammatory cells, etc.) can be analyzed. This technology provides an excellent means for identifying the key genes and proteins that are associated with tumor progression and will likely yield targets for the future diagnosis and treatment of cancer. (Webb and Monsma) 51

Immunofluorescent analysis of blood vasculature in the eye of an Lrp5-deficient mouse Mutations that inactivate the gene encoding the low-density lipoprotein receptor–related protein 5 (Lrp5) cause the human syndrome osteoporosis pseudoglioma (OPG). Patients suffering from this syndrome develop earlyonset osteoporosis and have vision problems due to inappropriate vascularization of the eye. We have found that Lrp5-deficient mice model both the osteoporotic and eye problems seen in humans. Shown is an immunofluorescence analysis of a paraffin-embedded eye section from an Lrp5-deficient mouse stained with antibodies to Factor VIII (red) and CD31 (green), two molecules associated with endothelial cells. The prominent vascularization is absent in normal mice of this age. (Williams) 52

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