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2005 Scientific Report

  • Text
  • Report
  • Institute
  • Mice
  • Proteins
  • Signaling
  • Protein
  • Michigan
  • Tumor
  • Molecular
  • Laboratory

Title page photo:

Title page photo: Podosomes in transformed cells This image shows podosomes in NIH3T3 mouse fibroblasts transformed with activated Src tyrosine kinase. Podosomes are the rounded structures at the tips of many of the cell extensions. They are rich in filamentous actin, which has been stained green with a conjugated phalloidin dye. The cells are co-stained with an antibody that recognizes the adhesion protein CrkL (red; or where co-localized with actin and its phalloidin stain, yellow). The protein CrkL is involved in integrin-induced cell adhesion and migration. The study of these proteins (Src, F-actin, CrkL, integrins) in these and other tumorigenic cell types may shed light on the mechanisms of podosome-mediated cancer cell metastasis and invasion. (Eduardo F. Azucena, Jr., Darren Seals, James Resau, and Sara Courtneidge) Published June 2005 ©2005 by the Van Andel Institute All rights reserved Van Andel Institute 333 Bostwick Avenue, N.E. Grand Rapids, Michigan 49503, U.S.A.

Contents Director’s Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Laboratory Reports Cell Structure and Signal Integration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Arthur S. Alberts, Ph.D. Antibody Technology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 Brian Cao, M.D. Mass Spectrometry and Proteomics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Gregory S. Cavey, B.S. Signal Regulation and Cancer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 Sara A. Courtneidge, Ph.D. Cancer and Developmental Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 Nicholas S. Duesbery, Ph.D. Vivarium and Transgenics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 Bryn Eagleson, A.A., RLATG Bioinformatics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 Kyle Furge, Ph.D. Cancer Immunodiagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 Brian B. Haab, Ph.D. Molecular Medicine and Virology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 Sheri L. Holmen, Ph.D. Integrin Signaling and Tumorigenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 Cindy K. Miranti, Ph.D. Analytical, Cellular, and Molecular Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 and Microarray Technology and Molecular Diagnostics James H. Resau, Ph.D. Germline Modification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 Pamela J. Swiatek, Ph.D., M.B.A. Cancer Genetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 Bin T. Teh, M.D., Ph.D. iii

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