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2018 Scientific Report

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Vivarium and Transgenics

Vivarium and Transgenics Core BRYN EAGLESON, M.S., LATG Ms. Eagleson earned an M.S. degree in laboratory animal science from Drexel University’s College of Medicine. She worked for many years at the National Cancer Institute’s Frederick Cancer Research and Development Center in Maryland before joining VARI as the Director of Vivarium and Transgenics in 1999. SERVICES The goal of the VARI Vivarium and Transgenics Core is to develop, provide, and maintain high-quality mouse modeling services. The vivarium is a state-of-the-art facility that includes a high-level containment barrier. Van Andel Research Institute is an AAALAC-accredited institution, most recently reaccredited in November 2016. All procedures are conducted according to the Guide for the Care and Use of Laboratory Animals. The staff provides rederivation, surgery, dissection, necropsy, breeding, weaning, tail biopsies, sperm and embryo cryopreservation, animal data management, project management, and health-status monitoring. Transgenic mouse models are produced on request for project-specific needs. The creation of gene-targeted mice using the CRISPR/Cas9 system has been implemented. We also provide therapeutic testing and preclinical model development services. Projects include pharmacological testing, target validation testing, patient-derived xenograft (PDX) development, orthotopic engraftment model development, and subcutaneous xenograft/allograft model development. The Small-Animal Imaging Facility provides preclinical imaging technologies that offer anatomic and functional information to biomedical investigators. Currently available technologies include high-resolution microCT, micro-ultrasound, and optical imaging. STAFF Megan Briggs, B.S. Brandon Bonnema, B.S. Stephen Bowman, M.S. Charles Bradfield, B.S. Rita Burdette Thomas Dingman, B.S. Nicholas Getz, B.S. Sara Greenwald, B.S. Audra Guikema, B.S., LVT Tristan Kempston, B.S. Tina Meringa, A.A. David Monsma, Ph.D. Lori Moon, E.M.B.A. Malista Powers, A.S., LVT Mathew Rackham Lisa Ramsey, A.S., LVT Adam Rapp, B.S. Yanli Su, A.M.A.T. Aurora Thoms, A.S. Collin Tidd, A.S. William Weaver, B.S. 52 | VAN ANDEL RESEARCH INSTITUTE SCIENTIFIC REPORT

Confocal Microscopy and Quantitative Imaging Core CORINNE ESQUIBEL, Ph.D. Dr. Esquibel has a B.S. in biology from Truman State University and a Ph.D. in molecular and cellular pharmacology from the University of Wisconsin–Madison. She joined Van Andel Research Institute as the Core manager in 2017. SERVICES Established in October 2013, the Core provides optical imaging services for Van Andel Research Institute and collaborating institutions. We focus on comprehensive training of users for every aspect of imaging: experimental design and optimization, data acquisition, and image analysis. This helps users of all experience levels to perform quantitative research at or exceeding the professional standards of their field. To do this, we maintain multiple instruments with a range of imaging capabilities. STAFF Kristin Feenstra, B.S. Lori Moon, E.M.B.A. A Nikon A1plus laser scanning confocal is an essential instrument within the Core, designed to generate high-resolution images in multiple dimensions. It is equipped for imaging in both galvanometric and resonant scanning modes with four solid state lasers, four high-sensitivity detectors, and a multi-anode spectral detector. The confocal can achieve optical sectioning of cells and tissue and can image live samples over time. The computer-coded stage allows for imaging large areas of the sample. The PerkinElmer Vectra 3.0 Automated Quantitative Pathology Imaging system is capable of imaging up to 200 slides per session, using a sophisticated multispectral camera to mathematically unmix up to seven fluorophores in each slide. Trainable algorithms in the PerkinElmer inForm software allow for automated segmentation and quantitative phenotyping of slides. Image analysis is supported not only through consultation and training of users, but also through the availability of a powerful Silicon Mechanics PC workstation that contains a suite of commercial and open-source image analysis programs. Analysis options include 3D-5D visualization (FIJI, Nikon Elements, Imaris), deconvolution (Huygens Professional), neuron tracing (Imaris), segmentation (Imaris, MATLAB), machine learning (CellProfiler and CPAnalyst), and figure preparation (FIJI/Image J, Illustrator, Photoshop). When out-of-the-box solutions are not available, additional sophisticated mathematical analysis can be written for case-specific applications (MATLAB). VAN ANDEL RESEARCH INSTITUTE SCIENTIFIC REPORT | 53

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